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Detekce a identifikace patogenu konopí setého (Cannabis sativa)
Jarošová, Natálie
The aim of this work was the detection and identification of Hop Latent Viroid in plants of Cannabis sativa. This Viroid, which may not have any symptoms on plants, as the name suggests. One of the few detection options is based on the principle of the polymerase chain reaction - PCR. Using the chloroform-phenol reaction, it was possible to isolate RNA of the Viroid, which was used as a template for cDNA and subsequently multiplied by the PCR method. After successful PCR, the samples were applied to an agarose gel and evaluated by comparison with a size marker. For subsequent testing of the pathogen detection and identification protocol, positive samples from Genetia BioSciences were added, and for testing real samples from the genotype collection from the Department of Plant Biology, plants with the presence and absence of Hop Latent Viroid were identified.

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